Description:Lipofectamine™ 2000 Transfection Reagent is a proprietary cationic lipid formulation that offers the highest transfection efficiencies and protein expression levels on the widest variety of adherent and suspension cell lines. A large View MoreLipofectamine™ 2000 Transfection Reagent is a proprietary cationic lipid formulation that offers the highest transfection efficiencies and protein expression levels on the widest variety of adherent and suspension cell lines. A large percentage of our customers also use this reagent for RNAi gene knockdown experiments. It is the leading transfection reagent on the market for simple, effective, and optimal nucleic acid delivery (Figure 1). With Lipofectamine™ 2000 Reagent you will get:
* Exceptional transfection efficiency in a broad range of cell lines and high levels of recombinant protein expression
* Superior performance for siRNA delivery (1) (Figure 2)
* Proven efficacy in the presence of serum-eliminates the need to change media following transfection
* A reagent proven to work in high-throughput applications
* A reliable reagent for establishing stable cell lines
Lipofectamine™ 2000 Reagent works effectively with many primary cell lines and other difficult-to-transfect cell lines (Figure 3, 4, 5). In addition to its versatility with diverse cell lines, Lipofectamine™ 2000 allows you to achieve high transfection efficiencies using media with or without serum. For cells sensitive to the absence of serum, add DNA: lipid complexes directly to cells in serum-containing medium. For gene silencing Lipofectamine™ 2000 Reagent's high-efficiency transfections leads to the high levels of gene knockdown needed to achieve successful results.Simply mix Lipofectamine™ 2000 Reagent with nucleic acid, add to cell culture, and express protein or knock down genes. The simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Reagent ideal for transient protein expression or high throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.Protocols, citations, and additional product information for Lipofectamine™ 2000 can be found in the Cell Lines Database at www.invitrogen.com⁄celllines.Contents and Storage:Each unit contains one vial of reagent. Store at +4°C. Lipofectamine™ 2000 Reagent is guaranteed stable for 6 months when stored properly.
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